Sunday, 10 November 2013

Final Post : Murder Most Brutal !

Finally we have come to our FINAL post on this investigation !!

After weeks of analysis, investigation and interrogation of several people, we are proud to say that we BRIGHTLIGHTS detectives have come up with a conclusion by putting together different pieces of the puzzles that are given to us.

So , let us begin :)

We have identified 3 victims , and that would be Anya Suriati who is 9 years old, Herman Hartono & his son, Adi Hartono. This is proven to be it as tests and investigations have been carried out. But what is funny is that how are these people related to begin with ?

Who is the main victim ? Is it Anya ? Herman ? Or Adi ?

We dug deeper into everything, all three of their histories, where they went, their family ties, friends, and everything we could think of. Finally , we managed to get hold of the wife of Herman, Sally who has been hiding far away in Philippines. After hours of questioning her, she told us her side of the story which finally connected the dots of everything and revealed the final truth.

It turns out that, Herman happens to be a pedophile. Yes, he was, and his wife knew about it and kept quiet about it for years. He has always been eyeing Tanya Rosilawati, who is the twin of Anya. One night, Sally and Herman got into a huge fight because Herman told her about his intentions to rape Tanya. Sally failed to stop him from doing it and Herman went on with his plans.

However, he mistakenly kidnapped Anya instead of Tanya, raped her and even cruelly killed her after. It was not his first time raping a person but Anya was unlucky because she could recognise him. Herman panicked after killing her , and being the heartless person he was, he decided to accuse and put all the blame onto his son.

His son, after finding out the truth, could not believe what his father had done. He also did not want to take the blame. While they were arguing things became bad and they started provoking each other. Eventually, they got into a huge fight. Herman accidentally killed his son out of anger. All these happened too quickly, and Herman could not believe what he did. He could not handle the guilt that was overwhelming him, so he then killed himself as well. His wife who witnessed the entire situation was traumatized, she didn't know what to do with the bodies. In the end, she buried both Herman and Adi together with Anya since Herman had told her where she was buried. Right after, Sally left hoping that no one would ever find out about everything. 

Hence, this concludes our entire investigation. The truth is finally revealed ! :D



Since this would be our last post on this investigation, we would like to thank everyone who has been reading and supported us throughout the process. & also, a big thank you to our fellow lecturers who gave us this opportunity to work and grow together as a team . 


Lastly, here's a picture of us investigators who have been working hard together for weeks :)) 

Best team mates everrrrr <3 !

THANK YOU , 

Signing off, 

xoxo,
BrightLights

Sunday, 3 November 2013

Part III : Murder Most Brutal

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Okay so we're back with two more questions this week! :D Let's get down to business? hehe 

Question 5 


Match the photos of the victims’ pieces of evidence, explaining your rationale for doing so. 


Here are the following people who were reported missing. 
  • Herman Hartono ( Father of Adi Hartono )
  • Adi Hartono ( Son of Herman Hartono )
  • Anya Suriati, 9 year old girl
Let's address the obvious evidence first.


From the picture above, we can see that the jaw is actually smaller compared to the rest of the evidence provided.So we can safely assume that..... It belongs to a female which is Anya Suriati! 

Okay now let's move on to the rest of the evidence ! 

 



As you can see from the above evidence.... the third molar has erupted already, so our first assumption will be that it belongs to Herman Hortono ... BUT! because the pictures only shows a section of the jaw thus, we cannot conclude anything because we're unsure as to whether the rest of the third molars have erupted or not and whether its the FIRST third molar to erupt. 

MOVING ON.... 

  

Looking at 48, it looks like it has not erupted. Again, we would jump to conclusions... assuming it belongs to Adi Hartono.... but LISTEN!(lol) the tooth might have been impacted or disfigured.... HENCE! It could also belong to his father, Herman Hartono! 


Question 6

The DNA profile showed evidence for a father and son. In addition there was DNA profiling pattern that matched one living twin (Tanya Rosilawati). With this, match the evidence to these victims who were earlier filed for missing persons. Explain your rationale.


Since the DNA results shows evidence of a father and a son, there is a high possibility that Herman Hartono and Adi Hartono may be the victims of the crime as both of them are reported missing together & they live a few blocks away. 
How DNA profile show evidence for father and son ?
Every child inherits half the chromosome from their parents, for example 23 from the mother and 23 from the father. This being the reason why there are two sets of alleles which n=46 for each person ; Biology is definitely a fascinating and wonderful science,  the inheritance of patterns of the markers being used does not have to correspond identically with one set of column of alleles of each parent but rather be a combination of allele from each parent. We test a system of 16 genetic markers which is more than sufficient to establish a 99.99% inclusion probability whether a alleged father is actually the father but 100% exclusion probability whether a alleged father is not the father . Since your DNA is the same throughout every cell in the body, paternity test can be determined using DNA samples collected just from every part of the body, in this case, the skull and the jaw fractions. Then PCR is carried out to give scientist the ability to billions of copies of DNA from just a small sample. As a final step in DNA testing for paternity, a combined relationship from all the alleles that were tested is tallied and it appears below with the chart. 

Next, there is also another DNA profiling pattern that matched a living twin, Tanya Rosilawati. This can confirm that the DNA profile must belong to Anya Suriati because identical twins share the same DNA profile. It is almost impossible to distinguish the difference of DNA profile between a pair of identical twins as they are monozygotic. 

What are monozygotic twins ? 

They are zygotes that are formed from a single fertilised zygote. This zygote divides into two individual zygote AFTER conception and undergo mitosis from then. This results in the development of two identical embryos consisting of the exact same DNA. They have remarkably similar physical characteristics. The only way to differentiate between a pair of identical twins is by their fingerprints pattern. This is because fingerprint is not entirely a genetic character but rather a phenotypic character - meaning the interaction of an individual's genes as well as the developmental environment in the uterus. 
The ultimate shape of fingerprints are believed to be influenced by environmental factors during pregnancy, like nutrition, blood pressure, position in the womb and the growth rate of the fingers at the end of the first trimester. Thus, you will find similar patterns of whorls and ridges in the fingerprints of identical twins. But there will also be differences -- just as there are differences between the fingers on any individual's hands.



--


& Thats it for today ! Bits and pieces coming together and we are slowly having a clearer picture and a proper direction as to how the investigation should go on. Cannot wait for the final part to be up next week and that's when we would come up with a conclusion with what exactly happened :D

Stay tuned .. :) 






Monday, 14 October 2013

Part II : Murder Most Brutal !

After a week of hiatus, we are back on the investigation with some new information and evidences ! :D Our group has come together and brainstormed to solve this mystery case, trying our very best to think like professional detectives :p It was indeed very challenging to push ourselves to pick up details, connecting the dots, and slowly figuring out and resolving the case together as a team :)



The following would be our findings and hopefully it is informative and helpful :) 


Question 4 

Other than examining the dental aspects provided as evidence, what other ways can the victims be identified?

There's a necessity to identify a deceased due to many incidents and at times, bones are the only accessible source for examination.  Forensic odontologists have done a great job in aiding the identification process.  A successful alternative is to use DNA technology for identification process.  Typically, this process involves the usage of short tandem repeat (STR) markers and fluorescent technologies, which are widely used in the field of forensics.  However, in certain aged and burned bones, DNA is highly degraded and the process for DNA extraction may become more complicated and handful.  The major challenges during the process is to maximize DNA yield, minimize the possibility of outside contaminants and obtain high quality DNA samples.



The other simpler way to aid the identification process is to create a biological profile for the victims.  There are few parts of the bones that can be examined to determine their age, sex, height and to a further extend, their occupation or maybe are they malnutrition or not.  The following aspects that should be examined :


  • Epiphyseal union - As a person ages, the areas of the femur gradually fuses together.  This can help to estimate young ages if the teeth are missing.
  • Pelvic girdle - Female's is always wider than male's.  This is the dramatic identifiers of sex of the victim.


pelvic girdle
  • Wrist - Bony ridges form where muscles were attached and pulled over years.  A forensic anthropologist may find a bony ridge on wrist and decide the victim may have been someone who used their hands for living, such as a chef or seamstress.  
  • Sternum - Examine the region where ribs join to sternum and compare it with a database of standard markers.  This is a good way to identify victim's age.
  • Skull - Examine the sagittal suture. If it is completely fused, the victim is likely to be of someone older than 35.  Then examine the coronal suture which fully fuses by age of 40



  •  Decomposition - By looking at the stage of decomposition, we can estimate the time of death of the victim. The stages of decomposition are :

1) fresh stage (three days after death)
2) putrefaction stage (between days 4 and 10)
3) black putrefaction stage
4) butyric fermentation stage
5) dry stage

have a look at the article from the link below to know more about this part of forensic science! :)





Question 5


Part 1

List the evidences that have been presented to both the doctors.

  • An adult skull with a large jaw bearing five teeth
  • A child's jaw with some teeth still in eruption
  • A piece of jaw bearing three back teeth.

Part 2

Considering the evidences available up to this point in time, describe in detail how you would go about investigating further, assuming you are Dr. Rudi or Dr. Suria, explaining what you anticipate at each step.


The tooth is the most valuable source to extract DNA since it is a sealed box preserving DNA from extreme environmental conditions, except its apical entrance. This has prompted the investigation of various human tissues as potential source of genetic evidentiary material. Moreover, when morphologically evaluated, even a single tooth provides valuable information regarding the individual to whom the tooth belongs.



If i were Dr Rudi or Dr Suria, i would first extract the DNA from the jaw pieces obtained from the scene and do a dental DNA fingerprintingDNA fingerprinting or DNA profile are encrypted sets of numbers that reflect a persons DNA makeup, which can also be used as the persons identifier. i would extract the DNA from the pulp of the teeth as the pulp produced the strongest PCR amplification signals. There are a few approaches i can take to sampling dental source DNA such as crushing entire tooth, conventional endodontic access, vertical splitting of the entire tooth, horizontal sectioning and cryogenic grinding. 

 I will then proceed on to analysing my DNA samples. Similarly i have a few methods of analysing to choose from, including Restricted Fragment Length Polymorphism (RFLP), Polymerase Chain Reaction (PCR) methods, Short Tandem Repeats (STR) analysis and also Y-chromosome STR (Y-STR) analysis for sex identification. Once i get the results, i will cross check the missing persons' list to see if i can get a match and find out if the victims are in any way related to each other. this will further narrow down my scope.

I could attempt on facial reconstruction as well to find out more about the victims involved in this case. Facial reconstruction is the method of reconstructing the living face of an individual from skeletal remains to aid identification ! How cool is that ? :O
Watch the video below to find out more!




If soil and plant samples are available, I would run a test on them as well. By sending the samples to the forensic botany lab, I can get an estimate of the time since death of an individual, connect a suspect to a victim or weapon or determine that a body has been moved.



Wednesday, 9 October 2013

Part 1 : Murder Most Brutal !





Question 1

We have all heard of how Forensic Dentistry has helped in the identification of burnt victims and also victims with bite marks. Explain more about these and describe how is Forensic Dentistry could be useful in the identification of murder victims?

Dental analysis is an excellent way to collect evidence and solve crimes. This is because teeth is able to withstand fire that destroys all kinds of evidence, burn human bones to ashes & even melt copper and glass ! :O



At the scene of the crime, odontologists collect the skull or remaining teeth, which are taken back to the forensic laboratory for the postmortem dental investigation. X-rays are taken and if the jaw is completely intact and the dental records used to compare are recent, the job of proving a match is a relatively simple one. Dentists mark on a chart the position of missing teeth, crowns, bridges, fillings, caps, root canals and various other treatments during a patient's routine check-up. 



It is said that no two people has the exact same dental imprint ! Using bitemarks, forensic odontologists are able to take bite-mark impressions on the skin of the victims. By comparing many factors, for example matching for striations whorls, indentations, pitting and abrasions, with the help of computer-enhanced photography.

The physical characteristics of both the bite mark wound and the suspect's teeth include:
• the distance from cuspid to cuspid
• the shape of the mouth arch
• the evidence of a tooth out of alignment
• teeth width and thickness, spacing between teeth
• missing teeth
• the curves of biting edges
• unique dentistry
• wear patterns such as chips or grinding.







It can also be determined from the type of bleeding beneath the skin whether the victim was alive or dead at the time the bite mark was delivered.



The forensic odontologist simply gives evidence as a witness to injuries caused by biting and it is the method in which they choose to present evidence in court that convinces a jury whether a suspect guilty or innocent.


Isn't it amazing how each and everyone of us are distinct in our own ways and we would not be able to escape from our wrong doings or wrongly accused ?! :O 


Question 2


Is a male human skull different from that of a female? If yes, how?




Yes,a male skull is different from a female skull ;

Weight : 

  • Male : Heavier 
  • Female : Lighter
Size : 
  • Male : Skull size is larger
  • Female : Skull size is lighter
Bony Mass / Thickness : 
  • Male : Thicker
  • Female : Thinner 

    Contour of the face : 
    Female Skull


    Male Skull
    • Male : Overall length of the skull is longer , chin is bigger & protrudes more forward ; Zygomatic                    bones are thicker
    • Female : Skull is rounded with the facial bones being smoother , jaws mandible & maxilla are smaller. 
    Forehead : 
    • Male : Slightly sloping or receding 
    • Female : Forehead is vertical 
    Vault of the skull :
    • Male : More rounded and prominent
    • Female : More flattened
    Supraorbital Margin : 
    • Male : More rounded
    • Female : Sharp 
    Frontal bone  : 




    • Male : Brow ridges are well demarcated.
    • Females : Smooth, more vertical frontal bone. 
    Mastoid Process :
    • Male : Larger Mastoid process

    • Female : Smaller Mastoid process


    So, this is just a video of us doing a simple comparison of a male and a female skull. Hopefully it helps to understand better ! Enjoy :)) 




    Question 3:

    From the story above so far, how many victims are there? Explain. 



    From our investigation, we assume there is only one victim because there was only one skull discovered and we assume the pieces of bone come from the same victim.


    Thats all for today ! Hope this has been informative :)


    The investigators ;) hehe.


    Stay tuned for more upcoming posts ! :D

    xoxo,
    Bright lights

    Tuesday, 24 September 2013

    WELCOME TO ALL TEETH LOVERS :))


    We are dental students from IMU and this blog is part of an assignment which contributes to our EOS exam
    So let us just introduce ourselves, the admins of this blog. :D


    From the left: ( Manju ), Vivian, Zhi Ying, Kate, Sze Yeen, Mabel, Chiam and Khei Qi :)


    There will be many upcoming posts on very interesting topics. Stay tuned :))



    Xoxo,
    Brightlights